EMAGE mouse gene expression spatial database

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EMAGE:7941

Trio triple functional domain (PTPRF interacting) ( MGI:1927230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013122. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013122_01	euxassay_013122_02	euxassay_013122_03	euxassay_013122_04

euxassay_013122_05	euxassay_013122_06	euxassay_013122_07	euxassay_013122_08	euxassay_013122_09

euxassay_013122_10	euxassay_013122_11	euxassay_013122_12	euxassay_013122_13	euxassay_013122_14

euxassay_013122_15	euxassay_013122_16	euxassay_013122_17	euxassay_013122_18	euxassay_013122_19

euxassay_013122_20	euxassay_013122_21	euxassay_013122_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7941_wholemount_strong.wlz

7941_wholemount_moderate.wlz

7941_wholemount_weak.wlz

7941_wholemount_possible.wlz

7941_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7941_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 04 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 15 16 17 18 weak expression: see section 03 04 08 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 17 weak expression: see section 08

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 17

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 14 15 weak expression: see section 12 13 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38693
Entity Detected:	Trio, triple functional domain (PTPRF interacting) ( MGI:1927230)
Sequence:	sense strand is shown >T38693 GTCCTTAAGGCATCCAGTATCGAGAACAAGCAGGACTGGATCAAGCACATCCGTGAGGTGATCCAGGAGA GGACGGTGCACCTGAGAGGAGCCCTGAAGGAGCCCATTCACATCCCCAAAACAGCTCCGGCAGCAAGGCA GAAGGGGAGGAGGGATGGAGAAGACCTGGACAGCCAAGGGGATGGCAGCAGCCAGCCCGACACCATTTCC ATCGCCTCCAGGACATCTCAGAACACACTGGACAGTGATAAGCTTTCTGGTGGCTGCGAGCTGACGGTCG TGATTCATGACTTCACAGCCTGCAACAGCAATGAGCTGACCATCCGGCGTGGCCAGACAGTGGAGGTCCT GGAACGGCCTCACGACAAGCCCGACTGGTGTCTGGTGCGCACCACTGACAGGTCCCCTGCAGCAGAAGGC CTCGTGCCCTGTGGCTCACTGTGCATCGCCCACTCCCGAAGTAGCATGGAGATGGAGGGCATCTTCAACC ACAAAGACTCACTGTCGGTCTCCAGTAATGACGCCAGCCCACCGGCCTCTGTGGCTTCCCTTCAGCCCCA CATGATCGGAGCACAGAGTTCACCAGGTCCCAAGCGGCCCGGCAACACCCTGCGCAAGTGGCTCACGAGC CCCGTGCGGCGGCTCAGCAGCGGTAAGGCCGATGGGCATGCGAAGAAGCTGGCCCACAAGCACAAGAAGA GCCGGGAGGTCCGTAAGAGCGCGGATGCTGGATCGCAGAAGGATTCGGATGACAGCGCTGCCACCCCGCA GGATGAGACCATCGAAGAGAGAGGACGGAACGAGGGTCTCAGCAGTGGTACGCTGTCCAAGTCCTCGTCC TCGGGCATGCAGAGCTGCGGAGAAGAAGAAGGCGAGGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161542. Forward Primer - name:161542_F_cDNA_mCG120315, sequence:GTCCTTAAGGCATCCAGTATCG; Reverse Primer - name:161542_N_SP6_cDNA_mCG120315, sequence:CCTCCTCGCCTTCTTCTTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7942 same embryo
	EMAGE:7943 same embryo
	EurExpress:euxassay_013122 same experiment
	MGI:4828922 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source