EMAGE mouse gene expression spatial database

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EMAGE:8024

Usp32 ubiquitin specific peptidase 32 ( MGI:2144475)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016000. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016000_01	euxassay_016000_02	euxassay_016000_03	euxassay_016000_04

euxassay_016000_05	euxassay_016000_06	euxassay_016000_07	euxassay_016000_08	euxassay_016000_09

euxassay_016000_10	euxassay_016000_11	euxassay_016000_12	euxassay_016000_13	euxassay_016000_14

euxassay_016000_15	euxassay_016000_16	euxassay_016000_17	euxassay_016000_18	euxassay_016000_19

euxassay_016000_20	euxassay_016000_21	euxassay_016000_22	euxassay_016000_23	euxassay_016000_24

euxassay_016000_25	euxassay_016000_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8024_wholemount_strong.wlz

8024_wholemount_moderate.wlz

8024_wholemount_weak.wlz

8024_wholemount_possible.wlz

8024_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8024_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 12 16 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 04 05 06 07 08 10 11 12 14 15 16 17 20 21

facial vii ganglion

weak

regional

weak expression: see section 05 06 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 09 18

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 08

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 13 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39568
Entity Detected:	Usp32, ubiquitin specific peptidase 32 ( MGI:2144475)
Sequence:	sense strand is shown >T39568 TGCTACCTCCTTTTTCCTGAAGTTTCATTCCGTTGTGTTACAAATGGAGAAAGCAAAACCATGCAGGAAT GCAGATAGTTCTTATGGAATGGACACAAAGAGTTTTCAGTTTTTTGAATCATTTCCCCTATCTCTCTGTG TCTTTCTTGCTTTTTGTTCTTGTTGGTTTTGTGTTTGAAACAGTGATTGGTGGCAGAGGCATATGACGGT CAGTCTGAACCGAAGAGTCTCTGAAGACTATGTGCCTTCTACCCACACTCAGTTCTGAGAACCAGGAGAA AGGAAAGGCAGCCATGGTGTCCAGCTCAGATGGCCCTCCTGTAGAGGCGCACTGCTGGGTGTGGCAGCTG CCACAGGGTTTGCACCATAGGTCCCAAGCTGATGTTGCACACAGCTTCTAGGAAGGTAGGCGGCTGCGAA GCCAGACTGTTTCCTGTTAGTGGTGGGATTGTTTTCCCCTTTGTTTCTCGTTTTGCACTTTAAAAGAGAG AACACATGCAAACAACTGTGCTTGTACTTTAATGGCTCTAAGGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87611. Forward Primer - name:087611_F_cDNA_Usp32, sequence:TGCTACCTCCTTTTTCCTGAAG; Reverse Primer - name:087611_N_SP6_cDNA_Usp32, sequence:GCCCTTAGAGCCATTAAAGTACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8027 same embryo
	EMAGE:8028 same embryo
	EMAGE:8026 same embryo
	EMAGE:8025 same embryo
	EMAGE:8029 same embryo
	EurExpress:euxassay_016000 same experiment
	MGI:4829136 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source