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EMAGE:805

Otx2 orthodenticle homolog 2 (Drosophila) ( MGI:97451)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:805
Fig 4B Bernier et al, 2001 [PMID:11641222] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:805Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
805_wholemount_strong_3D_1.wlz
805_wholemount_possible_3D_1.wlz
805_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:805_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain roof plate
detected detected
Expression in the pre-tectum
optic vesicle
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2153660
Entity Detected:Otx2, orthodenticle homolog 2 (Drosophila) ( MGI:97451)
Notes:The probe used in this study by Bernier et al, 2001 [PMID:11641222] is described as such: "the 900 bp Otx2 cDNA was a gift from Thomas Theil (D�sseldorf)". Probe ends points are undefined
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bernier et al, 2001 [PMID:11641222] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11641222] Bernier G, Vukovich W, Neidhardt L, Herrmann BG, Gruss P 2001 Isolation and characterization of a downstream target of Pax6 in the mammalian retinal primordium. Development (128):3987-94
Links:MGI:2153754 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI