EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:8351

Mycbp2 MYC binding protein 2 ( MGI:2179432)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009485. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009485_01	euxassay_009485_02	euxassay_009485_03	euxassay_009485_04

euxassay_009485_05	euxassay_009485_06	euxassay_009485_07	euxassay_009485_08	euxassay_009485_09

euxassay_009485_10	euxassay_009485_11	euxassay_009485_12	euxassay_009485_13	euxassay_009485_14

euxassay_009485_15	euxassay_009485_16	euxassay_009485_17	euxassay_009485_18	euxassay_009485_19

euxassay_009485_20	euxassay_009485_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8351_wholemount_strong.wlz

8351_wholemount_moderate.wlz

8351_wholemount_weak.wlz

8351_wholemount_possible.wlz

8351_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8351_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 03 04 05 06

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 19 moderate expression: see section 17

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 17 18 moderate expression: see section 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 15 17 18 19 moderate expression: see section 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 moderate expression: see section 16 17

trigeminal v nerve

strong

regional

strong expression: see section 07 08 13 14

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 15

cervical ganglion

moderate

regional

moderate expression: see section 09 15

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 13 14 15 16 17 18

neural retina

moderate

regional

moderate expression: see section 01 19 20 not examined expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36783
Entity Detected:	Mycbp2, MYC binding protein 2 ( MGI:2179432)
Sequence:	sense strand is shown >T36783 GTGAGAGTCACTGGTGGATGAAGGGCTCAACTCCTACTCAGATCTCAGAGATCATCATCAGGCTTATCAA GGATATGGCTGCAGGTCACCTGTCAGAAGCTTGGTCCCGAGTGACAAAAAATGCTATTGCAGAAACCATC ATTGCCTTGACCAAAATGGAAGAAGAATTTAGGTCTCCAGTGAGATGTATTGCAACAACTAGACTCTGGC TTGCCCTGGCATCGCTGTGTGTTCTTGATCAGGACCATGTGGACCGCCTCTCCTCGGGGAGATGGATGGG AAAGGACGGGCAACAGAAGCAAATGCCTATGTGTGACAACCATGATGACGGTGAAACTGCAGCTATCATC TTATGCAACATCTGTGGCAATCTGTGTACTGACTGTGACAGATTCCTTCATCTGCATCGACGAACGAAGA CTCACCAAAGACAGGTCTTCAAAGAAGAAGAAGAGGCTATAAAGGTCGACCTCCATGAAGGCTGTGGTAG AACAAAACTGTTCTGGTTGATGGCATTAGCAGATTCTAAAACAATGAAGGCAATGGTGGAATTCCGAGAA CACACAGGCAAACCCACCACGAGTAGCTCGGAAGCATGCCGCTTCTGTGGGTCCAGGAGTGGAACGGAGC TGTCTGCTGTTGGCAGTGTTTGTTCTGATGCAGACTGCCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96839. Forward Primer - name:096839_F_cDNA_Phr1, sequence:GTGAGAGTCACTGGTGGATGAA; Reverse Primer - name:096839_N_SP6_cDNA_Phr1, sequence:CTGGCAGTCTGCATCAGAACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8348 same embryo
	EMAGE:8347 same embryo
	EMAGE:8349 same embryo
	EMAGE:8350 same embryo
	EurExpress:euxassay_009485 same experiment
	MGI:4826533 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source