EMAGE mouse gene expression spatial database

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EMAGE:8468

Herc3 hect domain and RLD 3 ( MGI:1921248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007296. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007296_01	euxassay_007296_02	euxassay_007296_03	euxassay_007296_04

euxassay_007296_05	euxassay_007296_06	euxassay_007296_07	euxassay_007296_08	euxassay_007296_09

euxassay_007296_10	euxassay_007296_11	euxassay_007296_12	euxassay_007296_13	euxassay_007296_14

euxassay_007296_15	euxassay_007296_16	euxassay_007296_17	euxassay_007296_18	euxassay_007296_19

euxassay_007296_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8468_wholemount_strong.wlz

8468_wholemount_moderate.wlz

8468_wholemount_weak.wlz

8468_wholemount_possible.wlz

8468_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8468_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

brain

moderate

regional

moderate expression: see section 03 04 05 06 07 08 10 11 12 13 14 15 weak expression: see section 01 02 09 16 17 18 19 20

pituitary gland

strong

spotted

strong expression: see section 08 09 10 11 12 13

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 weak expression: see section 09

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 15 weak expression: see section 09 14

otic capsule

strong

spotted

strong expression: see section 05 06 07 14 15 16

pharyngo-tympanic tube

strong

spotted

strong expression: see section 01 02 moderate expression: see section 20

esophagus

moderate

regional

moderate expression: see section 11

meckel's cartilage

moderate

regional

moderate expression: see section 02 03 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 04 17

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

right lung

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1889
Entity Detected:	Herc3, hect domain and RLD 3 ( MGI:1921248)
Sequence:	sense strand is shown >T1889 CCCCTCTCCTCCCTTCCTCTTGCTCTCCTACTCTTCCTTGTCTTTGCTGCTTGACTCCGTTCCCCTCTTC CTTCTGGTAGTGGATGCATGCTGTCTTTTTTATTTTAAATTTTGTAAGTTTTTTTTTAAACCTCTGTCTC TAACTGTGAAAATATGAAGATACTCTTTATAATAGAGTTTTTGTTTTATCACATGTGAAGTGTGCATTTA CAGTGAAATTTCAGCAGGGGGATTATGTTAAGTCAAATGCGTGTGTCTCAAAAGTGACATGTTTAACTGC TCATCACTGTATAAGAAAATTCTACATGGTCAAAGAGTTCATGTAATTCTAATTTTAAATCTGTAGTAGA TAGAGAAAGTATTTATTTATCTAAAATGAAGTACTTATAGATTGTGATGCAGCACCGAGTCTTGATGAAG GAATGTAGATTTTTGCTTTTCCTGTTTTTGTTTTGAAAAGTTACTCCAATTGCTAAATTGGTAGTTTTTT TGTCCTTATAAATATAACATTTAAAAAAGA
Notes:	The probe template was PCR amplified from IMAGE:597752 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:597752 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8471 same embryo
	EMAGE:8467 same embryo
	EMAGE:8472 same embryo
	EMAGE:8469 same embryo
	EMAGE:8470 same embryo
	EurExpress:euxassay_007296 same experiment
	MGI:4825357 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source