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EMAGE:8743

Usp24 ubiquitin specific peptidase 24 ( MGI:1919936)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743
"Pseudo-wholemount" of euxassay_009940. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009940_01 euxassay_009940_02 euxassay_009940_03 euxassay_009940_04
EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743
euxassay_009940_05 euxassay_009940_06 euxassay_009940_07 euxassay_009940_08 euxassay_009940_09
EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743
euxassay_009940_10 euxassay_009940_11 euxassay_009940_12 euxassay_009940_13 euxassay_009940_14
EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743
euxassay_009940_15 euxassay_009940_16 euxassay_009940_17 euxassay_009940_18 euxassay_009940_19
EMAGE:8743 EMAGE:8743 EMAGE:8743 EMAGE:8743
euxassay_009940_20 euxassay_009940_21 euxassay_009940_22 euxassay_009940_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8743Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8743_wholemount_strong.wlz
8743_wholemount_moderate.wlz
8743_wholemount_possible.wlz
8743_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8743_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31090
Entity Detected:Usp24, ubiquitin specific peptidase 24 ( MGI:1919936)
Sequence:sense strand is shown

>T31090
AAGTGGCAGAGCTGGTCGGATGCTGGTGTTCTTACAGTTTTTGAATGTGAGGGACTAAAGTTGATTTTGA
ACACCTGGACACCCATCCCATTTTCTCCCTTCCCAGTCCACCACCTCTTCACACCCATGCTTATAGGAAG
ATTGTGTGGGACCCGCATTGAAACGCTGGCCTTGATACATGGAGGAATATCATTGCCAGCAACTGCACTC
AGGAGCCCGAATTCAGGAGTGAAATTGCCACTTCTAGTCAGCCCCTTATTTCCTATGGAAACGACGCCTT
CCGCCCTGCGCCTGCTGTCCTCACATAAAGCTGAATCAGGGTCGCCGACACTGTTCACGTTGGTCCATCT
TCTTCCTTTGCTGCTGCAGTGAGCATCCTTCCACGCCATCAGGAACCACTTGATTTCCAACAGCTTCAGT
CCACTAACCTTCTGAGGACTTTTTTAAATGTAGTACCACAAAGTGACAATTATGGCAGGCCTGCCTTGAA
AGAATTGTCATTTTTACTGCCAATTTTTGGATGAAGATGTTTTTATAATCTTTTACAGTGGTCTGCAATC
AAGCAGGAATTGCACAATTGACTCAATGCTATGTGTTCTCAAGGAGCTCCTTCAGTCTAGGCTGCCCTCC
GGACTGCCCTCCTGCCCCTAAGGCACCGGGACACACAGATGAGCCTCCCACAGGCAGCTCCTGGAGCTCC
CCAGGTGGCCTCTCATGGTCACCTACAGCTATGAGCTGTAAGGCCCGGAGCCTGAGCCACCAGACTCACC
TGGCTCGCCCCACCCTCCCTGCTAGCACCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1381175), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57346. Forward Primer - name:057346_F_IRAV41_e11_Usp24, sequence:AAGTGGCAGAGCTGGTCG; Reverse Primer - name:057346_R_SP6_IRAV41_e11_Usp24, sequence:TTGGTGCTAGCAGGGAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8744 same embryo
 EMAGE:8741 same embryo
 EMAGE:8740 same embryo
 EMAGE:8739 same embryo
 EMAGE:8742 same embryo
 EurExpress:euxassay_009940 same experiment
 MGI:4829133 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS