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EMAGE:8784

Oasl2 2'-5' oligoadenylate synthetase-like 2 ( MGI:1344390)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784
"Pseudo-wholemount" of euxassay_009979. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009979_01 euxassay_009979_02 euxassay_009979_03 euxassay_009979_04
EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784
euxassay_009979_05 euxassay_009979_06 euxassay_009979_07 euxassay_009979_08 euxassay_009979_09
EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784
euxassay_009979_10 euxassay_009979_11 euxassay_009979_12 euxassay_009979_13 euxassay_009979_14
EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784 EMAGE:8784
euxassay_009979_15 euxassay_009979_16 euxassay_009979_17 euxassay_009979_18 euxassay_009979_19
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euxassay_009979_20 euxassay_009979_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8784Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8784_wholemount_strong.wlz
8784_wholemount_moderate.wlz
8784_wholemount_weak.wlz
8784_wholemount_possible.wlz
8784_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8784_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 12 13 weak expression: see section 11
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31140
Entity Detected:Oasl2, 2'-5' oligoadenylate synthetase-like 2 ( MGI:1344390)
Sequence:sense strand is shown

>T31140
CAGGATGCCTGGGAGAGAATCGAGAGGTTCTTTCGGGAACAGTGCTTCCGTGATGAGCTGCTCCTGGACC
AAGAAGTCAGGGTGATTAAGGTGGTGAAGGGAGGCTCCTCGGGAAAGGGGACAACGCTGAACCACAGATC
TGACCAAGACATGATTCTGTTCTTAAGCTGCTTTTCCAGTTTCGAAGAGCAGGCGAGAAACCGGGAGGTC
GTCATCAGCTTCATTAAGAAGAGGCTGATTCATTGTAGCAGAAGCCTGGCCTACAACATCATTGTCCTTA
CCCACAGAGAGGGCAAAAGGACCCCTCGCTCCCTCACCCTAAAGGTTCAGTCCCGGAAGACTGATGACAT
TATTTGGATGGATATCCTCCCGGCTTACGATGCTTTGGGACCTATTTCCAGAGACTCAAAACCAGCACCG
GCAATCTACGAGACTCTGATAAGAAGTAAGGGCTACCCTGGTGACTTCTCACCAAGCTTCACAGAGTTAC
AGAGACATTTTGTGAAAACTCGCCCAGTTAAACTGAAGAACCTCCTCCGGTTGGTGAAGTTCTGGTACCT
GCAGTGCCTGAGACGTAAATATGGAAGAGGTGCAGTGTTGCCCTCAAAATATGCTCTGGAGCTGCTGACC
ATCTACGCCTGGGAGATGGGTACAGAGAGCAGTGATAGCTTCAATCTGGATGAAGGGTTCGTAGCCGTGA
TGGAGCTCCTCGTAAACTACCGCGACATCTGCATCTACTGGACCAAGTACTACAATTTCCAAAACGAGGT
CGTCAGGAACTTTCTGAAGAAACAGCTGAAGGGAGACCGGCCCATCATCCTAGACCCAGCTGACCCCACC
AACAACCTGGGAAGAAGAAAGGGATGGGAACAGGTGGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2646375), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 53002. Forward Primer - name:053002_F_IRAV44_a04_Oasl2, sequence:CAGGATGCCTGGGAGAGA; Reverse Primer - name:053002_R_SP6_IRAV44_a04_Oasl2, sequence:CAGCCACCTGTTCCCATC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8782 same embryo
 EMAGE:8783 same embryo
 EurExpress:euxassay_009979 same experiment
 MGI:4826845 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS