EMAGE mouse gene expression spatial database

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EMAGE:8848

Wdfy3 WD repeat and FYVE domain containing 3 ( MGI:1096875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010172. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010172_01	euxassay_010172_02	euxassay_010172_03	euxassay_010172_04

euxassay_010172_05	euxassay_010172_06	euxassay_010172_07	euxassay_010172_08	euxassay_010172_09

euxassay_010172_10	euxassay_010172_11	euxassay_010172_12	euxassay_010172_13	euxassay_010172_14

euxassay_010172_15	euxassay_010172_16	euxassay_010172_17	euxassay_010172_18	euxassay_010172_19

euxassay_010172_20	euxassay_010172_21	euxassay_010172_22	euxassay_010172_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8848_wholemount_strong.wlz

8848_wholemount_moderate.wlz

8848_wholemount_weak.wlz

8848_wholemount_possible.wlz

8848_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8848_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vagus x ganglion

moderate

regional

moderate expression: see section 09 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 10 19 20 21

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18 19

cervical ganglion

moderate

regional

moderate expression: see section 10 18 19

thoracic ganglion

moderate

regional

moderate expression: see section 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

neural retina

moderate

regional

moderate expression: see section 01 02 03 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31823
Entity Detected:	Wdfy3, WD repeat and FYVE domain containing 3 ( MGI:1096875)
Sequence:	sense strand is shown >T31823 TTTCAGTGTTGCCCACCCCTGATTGTTTCTTTAGAAGGATGCCGGTGTCCCTCCATTGCAGAAACTGCTT TTGCACAGTAGGGAGCGGCCCTGCTGCCTCTGGACGCTTTGCAACAGTGTTACTTTACACTTTTGGAAAG CAGCGCCCTCCTGTGGCCCACGACCCTCATTTCCCCATACCCAATGGCAGAGGTGTTCAAGACTTGAATA GAGGAATGTGTGTGTAGCCACTTGAAGCTTGCTCCACTGTCTGTCTAACCTGACATGCTGTGGGATGAAC GCTCTTAAACTTTCCAGCCCATAACTAGTAGATGGAAAGCAAACCCTCGCTCACTATGGCATTTCCTCTG GCAGCTGGTGCTGTGGGCAACTGTCTTCTTTCCATCAGGTTTTGGTTTTTGTTTTCTTTTCCCTTCTGCA GAAATCACAGAGACACTCGCACTGGGCACTGGGCTCACATCCCTTGGCTTCATTGCTCATCTGGCTGGCT GGCCCTCCGTGTTCGTGCCATTCTCCTTACCAATATACACTATGTATATACCTCCTGCGGCCTGCATTCT TTTTCTAAGTACCTCACAGGGCTCCAGCCTGACCCTCTGTTCACCCCTCGCCCCTCCACCCACTGCTCAT CCACGTACTCGACACACTCGTGTGTAGCGTGCAAGTGTGATGTCACGCTTTTGAAGACGAGAAGTAGCTG CCCGTAGCAGCCAGCCCTCTGGAGAATAGCAAAACAAATCTAGATAAGTTATTTTGCACTTTCTTATGTA TAAAGGTGGTAGAAACTTATTTTTGCTTTGTATCATTTAAATACATTTTGTTTTGGTAAACAGACTGTGT ATAAACTATTTACGCGGTTGACGCTGTTTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6515138), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59445. Forward Primer - name:059445_F_IRAV99_g04_Wdfy3, sequence:TTTCAGTGTTGCCCACCC; Reverse Primer - name:059445_R_SP6_IRAV99_g04_Wdfy3, sequence:AAAAACAGCGTCAACCGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8845 same embryo
	EMAGE:8844 same embryo
	EMAGE:8843 same embryo
	EMAGE:8847 same embryo
	EMAGE:8846 same embryo
	EurExpress:euxassay_010172 same experiment
	MGI:4829190 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source