EMAGE mouse gene expression spatial database

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EMAGE:9843

Cdk14 cyclin-dependent kinase 14 ( MGI:894318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007187_01	euxassay_007187_02	euxassay_007187_03	euxassay_007187_04

euxassay_007187_05	euxassay_007187_06	euxassay_007187_07	euxassay_007187_08	euxassay_007187_09

euxassay_007187_10	euxassay_007187_11	euxassay_007187_12	euxassay_007187_13	euxassay_007187_14

euxassay_007187_15	euxassay_007187_16	euxassay_007187_17	euxassay_007187_18	euxassay_007187_19

euxassay_007187_20	euxassay_007187_21	euxassay_007187_22	euxassay_007187_23	euxassay_007187_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9843_wholemount_strong.wlz

9843_wholemount_moderate.wlz

9843_wholemount_weak.wlz

9843_wholemount_possible.wlz

9843_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9843_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 11 13 14

medulla oblongata basal plate

strong

regional

strong expression: see section 14 15 16 17 moderate expression: see section 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 12 19

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 09 10 20 21 22

pons mantle layer

moderate

regional

moderate expression: see section 09 19 21

facial vii ganglion

weak

regional

weak expression: see section 06 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 21

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 09 21 weak expression: see section 10 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 11 12 13 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50017
Entity Detected:	Cdk14, cyclin-dependent kinase 14 ( MGI:894318)
Sequence:	sense strand is shown >T50017 AATCGGTCTCCATCCCTCTTTTGTACATTCCTTCCATGGGAAGTCTTTACAGGTATCTTGATTTAAAGTA TAATTAAATTGGGAGTTCTTTAAGTGGGGAGAAAACTTTATGTGTAATTAAAAACAGTCTCCCTGTGATT TGCTTAGAATCCTCCATACATCTGGGGACGGGGGAAGATTATGTTTGTTTTCACTAAGTAGAAGTCAGGA GCTCACAGGAATGCTGGGAGGGTTTCAGTAAGAACATAAAGGCAACCCCATGTCCTTGCCCTCGTCTAGA CAGCTTCTCCTGGGAAGCAAGACATAACACTGCTCAGTGTGCGGTCTGCTTCACGACAGGGACAGGCACA CCTCTCAAGTTTAAAGGGTTTCAGTTTCCTGTCCTTGTCACCAGTGAAGGAAATGCAAAGTGTCTGCCCC CACCCCTCCACCCAACACAGTCCCCCTTCTCTGGCTTTTGCTCTCCTGGCCTCTCCAAGGGTGTCATGAA AATCAGTATAGACTTTGTTTCATTAACATGTCATATGTTGAGAGAGCACTGGAATGTTTGGTGAGTTTAT TTTTCTATGAACGCGTAGCTTGAGTTTGGACAACTAGTAAGGAATGCACCAACACTTTGGCATGACAATT GTATGAAAGCAACCTCACTGCACAAAGTATTTGGAAAAGAAGTATGGGACTTAGTTTCTATCTTCTTAAG GGGAGCCGTTTAATTAAATACAACATCCATGGGACAGGAAATGTGTTTGCTATAAAATTAGAGATATAAG GCAGGGGCAGCAATCTGCCCACCATGCAAGGATGAAAATCTGTTTCCAGCTCTCAAGTGGCTAAAAGTTT CCAAGAGAGTCCGTGGGATTATAGGAAAACCAGGGCCTCTTGGTACCCTGTCCTCAGAGTGTGTGACAGC CACTGTAATCAGTGCTCTATGTATGCGTCCATAGCAGACACTCATCTGTGAATATCGCTGGGTTTGTAAT CTTTTGTTATATAAGAGGATGTTTAGGCTGTACATACTGGGGTAGATCATTGCCTGCCCCTATACAT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AATCGGTCTCCATCCCTCTT; Reverse Primer - name:unspecified, sequence:ATGTATAGGGGCAGGCAATG. The reverse primer contains a 5' extension containing an T7 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using T7 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9842 same embryo
	EMAGE:9840 same embryo
	EMAGE:9841 same embryo
	EurExpress:euxassay_007187 same experiment
	MGI:4823776 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source