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EMAGE:3940

Fzd2 frizzled homolog 2 (Drosophila) ( MGI:1888513)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:3940 EMAGE:3940 EMAGE:3940 EMAGE:3940 EMAGE:3940
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Fzd2 probeA
Entity Detected:Fzd2, frizzled homolog 2 (Drosophila) ( MGI:1888513)
Sequence:sense strand is shown

>Fzd2 probeA
GTTGAGTGTTTACGTGACATTCTATATCATAAGATATTGATAAGAATGTTTCTAACATGTTTCTTCCCCG
CCCCTGCACACAAATAAAAGTAAGGATTTTGCTAAGTTAGCGGCCTGAGAGATGTTCTATAAAGTATCTT
TGTTAGTGTGAGAATCCCAGTTTCCAGCAGGCATGGGTTCCCTCGAGCCTGGAGTACTTTACAGCTTGCA
TGCTAAAGAAGCGATCTGAAGACCAGTGTGCCAGAGCTCCGCTCTCAACCGTCTCTCCTGTTTCTTGATA
ATAAATAGTGCAAATTAAAACCTAATAATCATCATAATAAAGTGCATTTAATTATCTTCAGATCTAGCTC
TTTAATTGTGTTTAACAGGGTTGTAACACCACTGCCCTTAACCAAGCCACACCCCCTCCTCCCCTCTCCC
CTTCACCTACTTGGTTGGGGTTTGGGGAGAGTGGGCTCCAGGCCTCAATTGTTTGACTAAAGAGTTATGG
GAAAATTGATTTCTTATCATCTTTTGTTGGCTGACTTGGGGACAAGGGAGAAAGTAAGCTGGAACCCCTA
CTTCCAAGTGTTTGTGTGTGTTAGTTTATGTTGGGGGAAGGGAAGTGGAAATGTTTGGTGGTAATTGAAG
TCATATGTGGGGCTTTGCTTGCAATTGTATTAAAGCCTGGGCGTTTCAGCAGAGCCCCCCTTCCCAAAAT
ATA
nt 1227814 - nt 1228516 of NW_001030434.1
Notes:The Fzd2 probe used in this study was transcribed from the whole insert of IMAGE clone 775142, the entire sequence of which has been verified by Summerhurst and Murphy. Editors note: The insert of IMAGE:775142 covers the most 3' 339 nt of the Fzd2 cDNA and also includes 361 nt of adjacent genomic sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE