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EMAGE:5752

Ctnnb1 catenin (cadherin associated protein), beta 1 ( MGI:88276)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5752 EMAGE:5752 EMAGE:5752 EMAGE:5752 EMAGE:5752
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ctnnb1 probeB
Entity Detected:Ctnnb1, catenin (cadherin associated protein), beta 1 ( MGI:88276)
Sequence:sense strand is shown

>Ctnnb1 probeB
GAATGTCTGAGGACAAGCCACAGGATTACAAGAAGCGGCTTTCAGTCGAGCTGACCAGTTCCCTCTTCAG
GACAGAGCCAATGGCTTGGAATGAGACTGCAGATCTTGGACTGGACATTGGTGCCCAGGGAGAAGCCCTT
GGATATCGCCAGGATGATCCCAGCTACCGTTCTTTTCACTCTGGTGGATACGGCCAGGATGCCTTGGGGA
TGGACCCTATGATGGAGCATGAGATGGGTGGCCACCACCCTGGTGCTGACTATCCAGTTGATGGGCTGCC
TGATCTGGGACACGCCCAGGACCTCATGGATGGGCTGCCCCCAGGTGATAGCAATCAGCTGGCCTGGTTT
GATACTGACCTGTAAATCGTCCTTTAGGTAAGAAAGCTTATAAAAGCCAGTGTGGGTGAATACTTTACTC
TGCCTGCA
nt 2194 - nt 2621 of NM_007614.2
Notes:The template clone used to generate the Ctnnb1 probe used in this study was obtained from U.Borello & G. Cossu. The insert sequence of the clone has been verified by Vendrell, Summerhurst and Murphy (it spans nt 2194-2621 of the Ctnnb1 cDNA RefSeq NM_007614.2).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Victor Vendrell, Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Victor Vendrell, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE