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EMAGE:3837

Gpc3 glypican 3 ( MGI:104903)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3837 EMAGE:3837 EMAGE:3837 EMAGE:3837 EMAGE:3837
Raw signal output, as movie. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.
EMAGE:3837 EMAGE:3837 EMAGE:3837
Photograph prior to 3D imaging. Photograph prior to 3D imaging. Sample morphology following 3D imaging, as movie

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3837Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3837_wholemount_strong_3D_1.wlz
3837_wholemount_moderate_3D_1.wlz
3837_wholemount_weak_3D_1.wlz
3837_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3837_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Gpc3_T7
Entity Detected:Gpc3, glypican 3 ( MGI:104903)
Sequence:sense strand is shown

>Gpc3_T7
TTTGTCAAAGAAATCCATGCAGAGAAAGAGAAAAGAGGGAAACATGACAACCATGTTCCAACAGTCAAAT
GTAAGAAAATAATTTGGCACAACTTGAAGGTTTTTTTTTTCTTTGCAAAAAGACAATCTATCCTACCACT
AAAATTTACCATCCTCATAAAGATACCATTTGATTTTCGAAAACATAACACGCGGTTAGTCTCCTACTTA
ATGGTGGTGGGAGGAGGCATAAAAGATAAAAAAAAAAAAAAAAAGAAAAAAAAAAGAAGGTGGCTCCCTT
TCTGTAGGGACCACAGGTGCTGCAGGGCAGCACAGGCACTGGACGCTGGCAAGTCAGTGCACCAGGAAAA
AAAAGCACGCCACATAGATGGCCACACTGATGAGGATCTTCAGTGGGGACGGCATGTTCCCCACGCTGTG
AGAGGTGGTGATCTCGTTGTCCTTCTGATTTCCATGCTGCTTGTTCCCCGGAGCATCGTCCACATCCAGA
TCATAGGCC
nt 48517079 - nt 48517577 of NC_000086.5
Notes:These primers were used to generate the in vitro transcription template by PCR of mouse whole genomic DNA. They contain T7 or T3 promotor sequences and amplify a region corresponding to portions of the ORF and the 3'UTR. Forward primer: 5 AATTAACCCTCACTAAAGGGGCCTATGATCTGGATGTGG (T3 Sense) Reverse primer: 5 TAATACGACTCACTATAGGTTTGTCAAAGAAATCCATGCAG (T7 Antisense)
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Jacqueline Ramsay, David R FitzPatrick.
Principal investigator:David R FitzPatrick, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Submitted by:Jacqueline Ramsay, Medical Research Council, Human Genetics Unit MRC Western General Hospital Crewe Road, Edinburgh, Scotland EH4 2XU
Experiment type:screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceFACEBASE