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EMAGE:602

Tfap2a transcription factor AP-2, alpha ( MGI:104671)
TS11 (EHF Downs & Davies)
in situ hybridisation

Data Images
EMAGE:602

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Anterior is to the left, early headfold embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:602Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
602_wholemount__moderate.wlz
602_wholemount__notDetected.wlz
602_wholemount__possible.wlz
602_wholemount__strong.wlz
602_wholemount__weak.wlz
(what is wlz format?)
Download all expression domains: EMAGE:602_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
strong strong
regionalRestricted to lateral embryonic ectoderm.
body cavity or lining
not detected not detected
homogeneous
embryo endoderm
not detected not detected
homogeneous
mesenchyme
not detected not detected
homogeneous
embryo mesoderm
not detected not detected
homogeneous
notochordal plate
not detected not detected
homogeneous
notochordal process
not detected not detected
homogeneous
organ system
not detected not detected
homogeneous
primitive streak
not detected not detected
homogeneous
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9015
Entity Detected:Tfap2a, transcription factor AP-2, alpha ( MGI:104671)
Sequence:sense strand is shown

>MGI:9015
AGATCTTTAAGAGAAAAACTGGACAAGATAGGATTGAATCTGCCAGCAGGGAGACGTAAAGCTGCCAACG
TTACTCTCCTCACGTCACTAGTGGAAGGAGAAGCCGTCCACCTAGCCAGGGACTTTGGGTACGTGTGCGA
AACTGAATTTCCTGCCAAAGCAGTAGCAGAATTTCTCAACCGACAACATTCCGATCCCAATGAGCAAGTG
GCAAGAAAAAACATGCTCCTGGCCACAAAACAG
nt 13 - nt 255 of X57012.1
Notes:AP2 (Tcfap2a) probe covers bases 13-255 (a ~240bp BglII fragment) of clone lambda22 corresponding to coding sequence (see also Mitchell et al 1991 [PMID:1989904] ). Linearised with EcoRI and transcribed with T3.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:EHF Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ doi:10.1101/gad.5.1.105] [ PMID:1989904] Mitchell PJ, Timmons PM, Hebert JM, Rigby PW, Tjian R 1991 Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Genes Dev (5):105-19
Links:MGI:9015 same probe
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE