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EMAGE:6089

Matn1 matrilin 1, cartilage matrix protein ( MGI:106591)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089
"Pseudo-wholemount" of euxassay_003820. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003820_01 euxassay_003820_02 euxassay_003820_03 euxassay_003820_04
EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089
euxassay_003820_05 euxassay_003820_06 euxassay_003820_07 euxassay_003820_08 euxassay_003820_09
EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089
euxassay_003820_10 euxassay_003820_11 euxassay_003820_12 euxassay_003820_13 euxassay_003820_14
EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089
euxassay_003820_15 euxassay_003820_16 euxassay_003820_17 euxassay_003820_18 euxassay_003820_19
EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089 EMAGE:6089
euxassay_003820_20 euxassay_003820_21 euxassay_003820_22 euxassay_003820_23 euxassay_003820_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6089Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6089_wholemount_strong.wlz
6089_wholemount_moderate.wlz
6089_wholemount_weak.wlz
6089_wholemount_possible.wlz
6089_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6089_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23 24
radius
strong strong
regionalstrong expression: see section 24
ulna
strong strong
regionalstrong expression: see section 24
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 18 19 20 21 22 23 24
hand
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23 24
foot
strong strong
regionalstrong expression: see section 03 04
hindlimb digit 1
strong strong
regionalstrong expression: see section 06 07 08 09 20 21
hindlimb digit 2
strong strong
regionalstrong expression: see section 05 06 07 08 09 20 21
hindlimb digit 3
strong strong
regionalstrong expression: see section 05 06 07 08 09 19 20 21
hindlimb digit 4
strong strong
regionalstrong expression: see section 05 06 07 08 09 19 20 21
hindlimb digit 5
strong strong
regionalstrong expression: see section 05 06 07 08 09 20 21
knee joint primordium
strong strong
regionalstrong expression: see section 24
fibula
strong strong
regionalstrong expression: see section 01 02 21 22 23
tibia
strong strong
regionalstrong expression: see section 01 02 20 21 22 23
femur
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 22
nasal septum
strong strong
regionalstrong expression: see section 13
hyoid
strong strong
regionalstrong expression: see section 09 11 13 14 15
meckel's cartilage
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
cricoid cartilage
strong strong
regionalstrong expression: see section 09 10 11 12
thyroid cartilage
strong strong
regionalstrong expression: see section 08 09 10 11 12 13
axial skeleton
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15
cranium
strong strong
regionalstrong expression: see section 01 03 04 24
basioccipital bone
strong strong
regionalstrong expression: see section 02 03 04 05 18 19 20 21 22 23
basisphenoid bone
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
exoccipital bone
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 18 19 20 21 22 23
viscerocranium
strong strong
regionalExpression in the turbinate bone.
clavicle
strong strong
regionalstrong expression: see section 07 08 15 16
scapula
strong strong
regionalstrong expression: see section 01 02 03 04 19 20 21
sternum
strong strong
regionalstrong expression: see section 12 13
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 07 08 14 15
acetabular region
strong strong
regionalstrong expression: see section 06
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7798
Entity Detected:Matn1, matrilin 1, cartilage matrix protein ( MGI:106591)
Sequence:sense strand is shown

>T7798
GCAACCATGAAGGTCACCTCTGGTCCAGCCTTTGCTCTCTGTAGCCTGCTGCTCCTGCTGCTGCTGCTGC
TACAGGTCCCTGATAGCCTCAGTCTTGTCCCCCAGCCCAGAGGGCACCTCTGCCGGACACGACCCACGGA
CCTGGTGTTTGTTGTCGATAGCTCTCGAAGTGTGAGACCCGTGGAGTTTGAGAAGGTGAAGGTATTCCTG
TCTCAGGTCATTGAGTCACTGGATGTGGGGCCCAATGCCACGAGGGTGGGCTTGGTCAACTACGCCAGCA
CTGTGAAACCAGAGTTCCCGCTCCGGGCCCATGGCTCCAAGGCCTCGCTGCTGCAAGCTGTGCGCCGCAT
CCAGCCGCTGTCCACGGGTACCATGACTGGCCTGGCTCTGCAATTCGCCATCACCAAGGCCTTGAGTGAT
GCTGAGGGTGGACGCGCCAGATCCCCCGACATCAGCAAGGTTGTCATCGTGGTGACTGATGGGAGGCCCC
AGGACAGCGTGCGGGACGTGTCTGAGCGCGCGAGGGCCAGTGGCATTGAGCTGTTCGCCATCGGCGTGGG
CCGCGTGGACAAGGCTACGCTGCGACAGATCGCTAGCGAGCCGCAGGACGAGCACGTGGATTACGTGGAG
AGCTACAATGTCATCGAGAAGCTGGCCAAGAAGTTCCAAGAGGCCTTCTGCGTGGTGTCCGACCTGTGTG
CCACAGGGGACCATGATTGTGAGCAGCTGTGCGTTAGTTCTCCTGGCTCCTACACCTGTGCCTGCCATGA
GGGCTTCACCCTGAACAGCGATGGCAAGACCTGCAATGTCTGCCGTGGGGGTGGAAGCGGCTCAGCCACC
GACCTGGTCTTCCTCATCGACGGATCCAAGAGCGTGCGGCCTGAGAACTTTGAGCTGGTGAAGAAGTTCA
TCAACCAGATTGTGGACACGTTAGATGTGTCGGACAGGCTAGCCCAGGTGGGGCTGGTGCAGTACTCCAG
CTCCATTCGCCAGGAGTTCCCACTCGGCCGCTTCCACACCAAGAAGGACATTAAGGCCGCGGTGCGGAAC
ATGTCCTACATGGAGAAAGGCACCATGACTGGCGCCGCCTTGAAGTATCTCATAGATAATTCTTTCACTG
TGTCCAGCGGGGCAAGGCCTGGAGCCCAGAAGGTGGGCATCGTCTTCACCGATGGCCGGAGCCAGGACTA
CATTAATGACGCTGCCAGGAAGGCCAAGGACCTTGGCTTTAAGATGTTTGCGGTGGGCGTGGGCAATGCT
GTGGAGGAAGAGCTGAGGGAGATCGCTTCCGAGCCCGTGGCAGACCACTACTTTTACACAGCTGACTTCA
AGACCATCAACCAGATTGGCAAGAAGCTGCAGAAACAAATCTGTGTGGAGGAAGACCCCTGTGCTTGTGA
GTCCATACTGAAATTTGAGGCCAAGGTGGAGGGTCTGCTGCAGGCCCTGACCAGGAAGCTGGAAGCTGTG
AGCGGGCGGCTGGCTGTCCTGGAGAACAGAATCATCTAAGAACTCTGGTGGCCTGGCCACTTCCCACCCA
ATGCACAGTTCTTAGTGTCCTGGGAATGCCCCCTGTAGCTGGAGTTAGTCTGGTTTTGCTGCTTTAGCAA
GGGAACCTGCGGAGGACGGCTGCAGGTCTCCCCAGCTGTCCTGGGGCTGGGAGCAGGAGGGGACACATGC
TTGTGTCTGCTGGGAGTGTGTTATGATGGTGCCTGTGAGCATGTGTGTGCCTGATGGTGATCATGTGTGT
TTCAGAGCAAGGGTGTGTGCACAGGTGAGAACGCAGGGAAGAGGTGTGTGAGCGACTGCGTTCGTTATTT
TTCCAACCTAAAGCTTAATATATTCCTGTTTTTTTTTTCCTCCAGTTAATTCTTTACCCAGTCAGTTCTT
TACTCTGAGAGATTTTTTTTTTGTTTGTTTCTTGAGAAAAAAATAAAAACAATTAAATGTGGTTCGCTGT
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6315509 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6315509 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:17405 same embryo
 EMAGE:17409 same embryo
 EMAGE:17408 same embryo
 EMAGE:17407 same embryo
 EurExpress:euxassay_003820 same experiment
 MGI:4826111 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS