EMAGE mouse gene expression spatial database

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EMAGE:3941

Fzd3 frizzled homolog 3 (Drosophila) ( MGI:108476)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


3D reconstructed object showing signal.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

View mapped 3D expression image

Download individual expression domains:

3941_voxel_strong_3D_1.wlz

3941_voxel_possible_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3941_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Fzd3 probeA
Entity Detected:	Fzd3, frizzled homolog 3 (Drosophila) ( MGI:108476)
Sequence:	sense strand is shown >Fzd3 probeA GAATTCGGCACGAGAAGATGGAATCTGTGATTTGGGAATGCGGTTGATGGAGTTGCTATGCTGGCCAGAT GTGCCCAATGTAATAAAATGAAAAGAAGATACAAGATGATGTCATCTTCCCATATTGTGAAACCAAAAAC AAATGCCCTTTGTGAGACCAGGTTACCAGTTCTTTGACAGTACAGGGAGTTTTTAAACTGAGGAGCCTAA CAGATAAGGGGTACTTTCAAGCTGAGACCTGCAGGCATATACTGATCTAAAACGCATCTTGTGTAGATCT GATCATCCGAGCCTCATTCTGATCCAGGAAGAATGGCTGTGAGCTGGATTGTCTTTGATCTTTGGCTCTT GACTGTGTTTCTGGGGCAGATAGGTGGGCACAGTTTGTTTTCTTGTGAACCTATAACCTTGAGGATGTGC CAAGATTTGCCTTACAATACTACCTTCATGCCTAATCTTCTGAACCATTATGACCAACAGACTGCAGCTT TAGCAATGGAGCCCTTCCACCCTATGGTGAACCTGGATTGTTCTCGGGATTTTCGGCCATTTCTTTGTGC ACTCTATGCCCCTATTTGTATGGAATATGGACGTGTCACACTTCCCTGCCGTAGGCTGTGTCAGCGTGCC TATAGCGAGTGTTCAAAACTCATGGAGATGTTTGGTGTCCCGTGGCCTGAAGATATGGAGTGCAGTAGGT TTCCAGATTGTGATGAGCCATATCCCCGACTTGTGGATTTGAATTTAGTTGGAGATCCAACTGAAGGAGC CCCAGTTGCAGTGCAGAGGGACTATGGTTTTTGGTGTCCCAGAGAGTTAAAAATTGATCCTGATCTTGGC TATTCCTTTCTGCACGTGCGAGATTGTTCGCCACCATGTCCCAATATGTACTTCAGGAGAGAAGAACTGT CATTTGCTCGCTATTTCATAGGCCTGATTTCAATCATTTGCCTCTCTGCCACATTGTTTACTTTTTTAAC CTTTCTAATTGACGTCACAAGATTCCGTTACCCTGAAAGACCTATCATATTTTATGCAGTCTGCTACATG ATGGTGTCATTAATTTTCTTCATTGGGTTTTTGCTGGAGGACCGAGTAGCCTGCAATGCATCTAGCCCTG CACAGTATAAGGCTTCTACAGTGACACAAGGATCTCACAATAAGGCCTGTACCATGCTCTTTATGGTACT ATATTTTTTCACTATGGCTGGCAGTGTATGGTGGGTAATTCTTACCATCACATGGTTTTTAGCAGCTGTG CCAAAGTGGGGCAGTGAAGCTATTGAGAAGAAAGCATTGCTGTTTCATGCCAGTGCCTGGG
	nt 1 - nt 1321 of NM_021458.1
Notes:	The Fzd3 template used to generate the probe in this study was obtained from U. Borello/C. Cossu. The full insert sequence of this clone has been verified by Summerhurst and Murphy (it spans nt 1-1321 of the Fzd3 cDNA RefSeq NM_021458.1).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Kristen Summerhurst, Paula Murphy
Principal investigator:	Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:	Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:	non-screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source