Type: | in situ hybridisation probe |
Identifier: | Wnt3a probeA |
Entity Detected: | Wnt3a, wingless-related MMTV integration site 3A ( MGI:98956) |
Sequence: | sense strand is shown
>Wnt3a probeA
AGAGCCAGGGTTGTTTCAGGACCCATCTGATTCCCCAGAGCCTGCTGTTGAGGCAATGGTCACCAGATCC
GTTGGCCACCACCCTGTCCCGAGCTTCTCTAGTGTCTGTCTGGCCTGGAAGTGAGGTGCTACATACAGCC
CATCTGCCACAAGAGCTTCCTGATTGGTACCACTGTGAACCGTCCCTCCCCCTCCAGACAGGGGAGGGGA
TGTGGCCATACAGGAGTGTGCCCGGAGAGCGCGGAAAGAGGAAGAGAGGCTGCACACGCGTGGTGACTGA
CTGTCTTCTGCCTGGAACTTTGCGTTCGCGCTTGTAACTTTATTTTCAATGCTGCTATATCCACCCACCA
CTGGATTTAGACAAAAG
|
| nt 2310 - nt 2676 of NM_009522.1 |
Notes: | The Wnt3a probe template used in this study was generated by RT-PCR from 9.5 embryo mRNA using the following primers: forward: 5'-AGAGCCAGGGTTGTTTCAGGA-3'; reverse: 5'-CTTTTGTCTAAATCCAGTGGTGGG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). Probe was transcribed from the entire insert. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |