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EMAGE:3954

Wnt4 wingless-related MMTV integration site 4 ( MGI:98957)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:3954 EMAGE:3954 EMAGE:3954 EMAGE:3954 EMAGE:3954
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:3954Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3954_voxel_strong_3D_1.wlz
3954_voxel_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3954_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt4 probeA
Entity Detected:Wnt4, wingless-related MMTV integration site 4 ( MGI:98957)
Sequence:sense strand is shown

>Wnt4 probeA
CTTGACACACATGCGGGTGGAGTGCAAGTGTCACGGGGTGTCGGGCTCCTGCGAGGTAAAGACGTGCTGG
CGTGCTGTACCGCCCTTCCGCCAGGTTGGCCACGCGCTAAAGGAGAAGTTTGACGGTGCCACGGAGGTGG
AGCCACGACGCGTAGGCTCCTCCCGGGCGCTGGTGCCTCGGAATGCACAGTTCAAGCCACATACAGATGA
GGACCTGGTATACCTGGAGCCTAGCCCGGACTTCTGTGAGCAGGACATCCGCAGTGGCGTGCTAGGCACG
AGGGGCCGCACGTGCAACAAGACATCTAAAGCCATTGACGGCTGCGAGCTACTGTGCTGTGGCCGCGGCT
TCCACACAGCGCAAGTGGAGCTGGCCGAGCGCTGTGGCTGCAGGTTCCACTGGTGCTGCTTCGTCAAGTG
CCGGCAGTGCCAGCGGCTCGTGGAGATGCACACGTGCCGGTGA
nt 639 - nt 1101 of NM_009523.1
Notes:The template used to generate the Wnt4 probe used in this study was obtained from A. McMahon. Probe was transcribed from the entire insert. The insert sequence of the clone has been verified by Summerhurst and Murphy (it spans nt 639-1101 of the Wnt4 cDNA RefSeq NM_009523.1).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE