EMAGE mouse gene expression spatial database

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EMAGE:3955

Wnt5a wingless-related MMTV integration site 5A ( MGI:98958)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


3D reconstructed object showing signal.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

View mapped 3D expression image

Download individual expression domains:

3955_voxel_strong_3D_1.wlz

3955_voxel_possible_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3955_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Wnt5a probeB
Entity Detected:	Wnt5a, wingless-related MMTV integration site 5A ( MGI:98958)
Sequence:	sense strand is shown >Wnt5a probeB GAATTCCTCGGCCGCCTTCGCTCGGGTGGCGACTTCCTCTCCGTGCCCCCTCCCCCTCGCCATGAAGAAG CCCATTGGAATATTAAGCCCGGGAGTGGCTTTGGGGACCGCTGGAGGTGCCATGTCTTCCAAGTTCTTCC TAATGGCTTTGGCCACGTTTTTCTCCTTCGCCCAGGTTGTTATAGAAGCTAATTCTTGGTGGTCTCTAGG TATGAATAACCCTGTTCAGATGTCAGAAGTATATATCATAGGTGCACAGCCTCTCTGCAGCCAACTGGCA GGACTTTCTCAAGGACAGAAGAAACTCTGCCACTTGTATCAGGACCACATGCAGTACATTGGAGAAGGTG CGAAGACAGGCATCAAGGAATGCCAGTACCAGTTCCGGCATCGGAGATGGAACTGCAGCACAGTGGACAA TACTTCTGTCTTTGGCAGGGTGATGCAAATAGGCAGCCGAGAGACGGCCTTCACGTACGCGGTGAGCGCA GCTGGGGTGGTGAACGCCATGAGCCGAGCATGCCGGGAGGGCGAGCTGTCTACCTGTGGCTGCAGCCGCG CTGCGCGCCCCAAGGACCTGCCTCGGGACTGGTTGTGGGGCGGCTGCGGAGACAACATCGACTATGGCTA CCGCTTCGCCAAGGAGTTCGTGGACGCTAGAGAAAGGGAACGAATCCACGCTAAGGGTTCCTATGAGAGC GCACGCATCCTCATGAACTTACACAACAATGAAGCAGGCCGTAGGACAGTATACAACCTGGCAGATGTAG CCTGTAAGTGTCATGGAGTGTCTGGCTCCTGTAGCCTCAAGACGTGCTGGCTGCAGCTGGCGGACTTCCG GAAGGTGGGCGATGCCCTCAAGGAGAAGTATGATAGCGCGGCGGCCATGAGGCTCAACAGCCGGGGCAAG CTGGTGCAGGTCAACAGCCGCTTCAACTCCCCGACCACGCAGGACCTGGTCTACATCGACCCCAGTCCGG ACTACTGTGTGCGCAACGAGAGCACTGGCTCGCTGGGCACGCAGGGACGCCTGTGCAACAAGACCTCAGA GGGGATGGACGGCTGCGAGCTCATGTGCTGTGGGCGTGGCTATGACCAGTTTAAGACAGTGCAGACCGAA CGCTGTCATTGCAAGTTTCACTGGTGCTGCTATGTCAAATGCAAGAAGTGCACGGAGATTGTGGATCAGT TCGTGTGCAAATAGTGGTGTGCCTGCCCTTCACCCAGTCCCACTCCCAGGACCCACTTATTTATAGAAAG TACAGTGCTTCTGGTTCTTTTTATTTCTCCCCCAAGAATTGCAGCTGGAACCATGTGTTTTGTTTTGTTT TATTTTGTTTTTTCTTTTCTGTTACCATCTAAGAACTCTGTGGTTTATTATTAATATTATAATTAATATT TGGCAATAGTGGGGGAAACTAAGAAAAATATTTATTTTGAGGATCTTTGCAAAGTTAGTACAAAATTTCT TTCTTCTGATGCTACAGGATAAAGGGGAAAAACTATGTATTCGAACTTAGCTGTGCAGTTGGGGGTTCAC ATCTAGAAGGTGTAGGAGCCATTTTCTTCTCAAACAGAGAGTCCTTTGAGATGGGTGGTATCCAGGTGAA GGAGGAGGTACAGACCCATGAATAACAGTTCCTGTGACCAAAATGAATTGCAGGTGCTCTGGTACAAAAG ATCTTAAATATAGATATATTAAATATACATATATGCCAAAAATACAGAATATGAGACACTCCCTAACCCA GAGGTTACCAGCCTGGTTTTGTGGGTTTTTTGTTTTGTTTTGTTTTTTCTTTTTTTGGGTTTTGTTTGTT TGTTTGTTTGTTTGTATTTTTGGTGTGTGTGTGTGTGTATTTCTAGAATGATCTTTTAGAAGGTACAAGC AAGAATCTCATATCTTCAGAAGCAGGCATATCATGTATGTTACTGTGTCCCACCTACAGATACTCCATTC ATGAATGGGCCTTTTTCTAACAGTTCATGAATATTGGGGAGCCGGTGGGCTGGGGGAGGGAGGTCCCCAG AAATTAGAAAACTTGAAGTTTCCTACATTGAGGCCATAATCTTGTGTTAGCCCAGCTGATTCTTAATACC AGACTTTTAGATCCATAAAGGAATTTTTGACTAAAAAAA
	nt 576 - nt 2714 of NM_009524.2
Notes:	The template used to generate the Wnt5a probe used in this study was obtained from A. McMahon. Probe was transcribed from the entire insert. The insert sequence of the clone has been verified by Summerhurst and Murphy (it spans nt 576-2714 of the Wnt5a cDNA RefSeq NM_009524.2).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Kristen Summerhurst, Paula Murphy
Principal investigator:	Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:	Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2006.12.002] [ PMID:17280871] Miller SF, Summerhurst K, Runker AE, Kerjan G, Friedel RH, Chedotal A, Murphy P, Mitchell KJ 2007 Expression of Plxdc2/TEM7R in the developing nervous system of the mouse. Gene Expr Patterns (7):635-44
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source