Type: | in situ hybridisation probe |
Identifier: | Wnt9a probeA |
Entity Detected: | Wnt9a, wingless-type MMTV integration site 9A ( MGI:2446084) |
Sequence: | sense strand is shown
>Wnt9a probeA
TGTCTTCTTCAGAGAGCCAAACCACTAGGAGAGGGCAGCCACTCCATCCCCAGGGTGCAGCCTCCGGCCC
TCTGACCCTGAGCACTTCCGTGGAGTATCAGTGACTTTTAAATTATTATTATTATTTAACTAATATAGTA
ATTATTATTTTCCCTGTCCCCTTACCTCCTCATCTGTGGCTTTTCCCTCTGAGCTGCCCCAGGCCCAGTC
AAGCCAGTCTGGGAGGCCTTGCCTTCCCTCTCTTCATCGTCCCCCCCTCCTTTGCTTGCTCTAGTTTGAA
ACCACTAAATCAAGGTCATGTTTGGAAAGAAATGAAGCGATAGAGCCTAGCCATGGCCCACAAGGCTTCT
GGAATCCACTCCCCAATTCTACCTTCCCCAGGGTCAGAGACCCTTGGTGGTCCCTGCTCTGTGCCCTGCA
GGTGCAGAGATCAGGTTGCTGGATATAGATGGGGGATTTGGGGGTCCTGGAGCCATGGGCACGGAGACAG
GGGCAGGGTCAGCTTTTTGATTGTCCATCTCAGGAAGCCATTCTTGCTCTGTGTCCCCAA
|
| nt 1698 - nt 2247 of NM_139298.2 |
Notes: | The Wnt9a probe template used in this study was generated by RT-PCR using the following primers: forward: 5'-GTCTTCTTCAGAGAGCCAAACCAC-3'; reverse: 5'-TGGGGACACAGAGCAAGAATGG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). Probe was transcribed from the entire insert. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |