EMAGE mouse gene expression spatial database

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EMAGE:3963

Wnt9b wingless-type MMTV integration site 9B ( MGI:1197020)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


3D reconstructed object showing signal.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

View mapped 3D expression image

Download individual expression domains:

3963_voxel_strong_3D_1.wlz

3963_voxel_possible_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3963_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Wnt9b probeA
Entity Detected:	Wnt9b, wingless-type MMTV integration site 9B ( MGI:1197020)
Sequence:	sense strand is shown >Wnt9b probeA TCCTTCTCTATGACCCTAATCCTGCTCTGGCTCGAGGGTACAAGGCAAGAATGGAGCCTGGCAAAACTTG GGGACTAGAACACCTGGACCTACAGCCAAATCACCTGTACCCTGACTCTATGGCCAGGAGGGCCAGGGGT GGAGGAGGGTTAAAGATGAACTTGAAGTTGAGGGCTGAGGCTGACCAACCATTAAGACTGGTGCCTTAAG GCACCCTCAGTCAGGTCCTCTCCCTCCCTTCTCCATTCTTTCTCCAAGGCCCCGTTCCCCCTAAAATCCC ACCATAGCCATGCTGGGTCCCCCCTTCCCCCACACTGGGAACTTTAAGGAAGATATTCACAGGGTATTTC TGCCTACCTCATACATGTAATTTTCAAAAAAAATTAATTTATATAGTTAAGATATATGGGAAAGTATTTA TGTTATTTATATATCTTCTCTATTTCCTGGGCACCATATGGGGGGTTGTGTGTTTACCCAGAAGCCTCTG AGGAAACATGGCTGGGTCTGTCTGGGGCCTCGCAGAGCTGGATGCGCATAGCTGAGAGGTCACAGCTCCT GTGTCTCACTGTCTTGGAGCTCGGGAAGCACATGTACCTCCTGAGATAAACCCCGTGACACCAA
	nt 2833 - nt 3456 of NM_011719.3
Notes:	The Wnt9b probe template used in this study was generated by RT-PCR using the following primers: forward: 5'-TCCTTCTCTATGACCCTAATCCTGC-3'; reverse: 5'-TTGGTGTCACTTTTTTTTATCTC-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). The probe was transcribed from the entire insert.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Kristen Summerhurst, Paula Murphy
Principal investigator:	Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:	Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:	non-screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source