EMAGE mouse gene expression spatial database

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EMAGE:3964

Wnt10a wingless related MMTV integration site 10a ( MGI:108071)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


3D reconstructed object showing signal.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

View mapped 3D expression image

Download individual expression domains:

3964_voxel_strong_3D_1.wlz

3964_voxel_possible_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3964_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Wnt10a probeA
Entity Detected:	Wnt10a, wingless related MMTV integration site 10a ( MGI:108071)
Sequence:	sense strand is shown >Wnt10a probeA GGGGGGTTGCGCCCCAGAACTTCGGCCTCCAGTCCCCAGCCCGCTGCACCTCCTTACCCTCTAGAGGCCC CCTCCCCCTTACCCTCTAGAGGCACCAGGAGTTGTCGCAAGGGGCCCTTGGGAAATTCCCTGGACCCCTG TGCCAGGAGGTGCCCGGTTCGCCCGCTCCCCATCCACCCCCCCGAGGGCGGTGCCCGGGGGCGCTGCCCC ATGGAGCGGGGAGGCGGGCGCCGTCTGCTGCGGGAGCTGTGACCTGAGTAGGAGCTGTGTGTCGCAGCCG CCCCACCCCTGCCGATCATGCGCCGGCGACCCTGGTTCGCCAGTCCCACTGGGCTGTGAGCCCCCCACTC CTGGCCTGTCACGGCCCGCGCGCCATGGGCAGCGCCCACCCTCGCCCCTGGCTGCGGCTCCCACAAGGGC CCCAGCCGCGGCCTGAGTTCTGGGCGCTCCTGTTCTTCCTACTGCTGCTGGCTGCCGCTGTGCCCAGGTC AGCACCCAACGACATCCTGGGCCTCCGCCTACCCCCAGAGCCCGTGCTCAACGCCAACACAGTGTGCCTG ACATTGCCCGGCCTGAGCCGGCGGCAGATGGAGGTGTGTGTGCGTCACCCTGACGTGGCCGCCTCTGCTA TCCAGGGCATCCAGATCGCCATCCATGAGTGCCAGCATCAGTTCCGGGACCAGCGCTGGAACTGCTCCAG CCTGGAGACTCGGAACAAAGTCCCCTACGAGAGCCCCATCTTCAGCCGAGGTTTTCGAGAGAGTGCTTTC GCCTACGCCATAGCAGCTGCCGGGGTGGTGCACGCAGTGTCCAACGCGTGCGCTCTGGGTAAACTGAAGG CTTGCGGTTGCGACGCCTCCAGACGTGGGGACGAAGAAGCTTTCCGTCGGAAGCTGCACCGCTTGCAGCT GGACGCGCTGCAGCGCGGAAAGGGCTTGAGCCACGGGGTCCCTGAACACCCGGCCATACTTCCTGCCAGC CCAGGTCTGCAGGACTCCTGGGAGTGGGGTGGCTGCAGTCCGGATGTGGGCTTCGGAGAACGCTTCTCTA AGGACTTTCTGGACTCCCGAGAGCCTCACAGAGACATCCATGCTCGAATGAGACTCCACAACAACCGTGT GGGCCGGCAGGCGGTGATGGAGAACATGCGGCGTAAGTGCAAATGCCACGGCACCTCAGGCAGCTGCCAG CTCAAGACCTGCTGGCAGGTGACGCCTGAGTTCCGCACAGTAGGGGCGCTGCTGCGCAACCGCTTCCACC GCGCCACGCTCATCCGGCCGCACAACCGCAACGGTGGCCAGCTGGAGCCCGGCCCCGCGGGAGCACCCTC GCCAGCACCGGGCACTCCAGGGCTGCGCCGCAGGGCCAGCCACTCCGACCTGGTCTACTTTGAGAAATCT CCCGACTTCTGTGAGCGCGAGCCGCGCCTGGACTCGGCAGGCACTGTGGGCCGCCTGTGCAATAAGAGCA GCACGGGTCCCGATGGCTGCGGCAGCATGTGCTGTGGCCGCGGCCACAACATTCTGCGCCAGACGCGCAG CGAGCGCTGCCACTGCCGGTTCCACTGGTGCTGCTTCGTGGTCTGCGAAGAATGCCGCATCACCGAGTGG GTCAGCGTCTGCAAGTGAGCAGACCCAAGCTCCTCTGGGTCTCAAGAATGGTTGTCCTCTTGGTGCCTGG CTTCTGCCGCTAGCGGATCTGAGCCAGGCAGCAAGCAGCAGCCTTGGCTCCTGAGAGAGGTGGTTGGCTC TTACAGCCCCGAGGGTCTACAATCACCAGACAGTCCAGATCTGATTGACATTCCTCCGCTCACCTCTGTA GGTTCCCCTCTTTCTGTTCCTAGCTCAGACAGCTGGGGGTGATAGTGGAGACTGTTCCACACCCTAGGAC AGGTCACCAAAGCAGCCCAGCCTGGCATGCCTACCTCCTGTCATCTCTTCTTCCCTTCCCCAGGAGTGAT AGGCAATGCACTGAAGCTGATGGGCACCGGGGAAGAAAACTAAAAGGCAGAAATGGCCGTCATCGGGCTG AAGTGACTCTAAGGGCTCCAGACCTCTGCTCCTGTCTTTCACTTAACAGATATTTATTTTTGCGCTCTCT TTGAGACACTCTCTGGGGAAAAAGAAGCTCCGGAGTCTACAGGCTGATTAAGGGACATGGACAATAAACC AGTAAACACAC
	nt 289 - nt 2469 of NM_009518.1
Notes:	The Wnt10a probe used in this study was transcribed from the entire insert IMAGE clone 3976222, the sequence of which was verifed by Summerhurst and Murphy (it extends from nt 289 to 2469 relative to the Wnt10a cDNA RefSeq NM_009518.1).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Kristen Summerhurst, Paula Murphy
Principal investigator:	Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:	Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:	non-screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source