Type: | in situ hybridisation probe |
Identifier: | Wnt10b probeA |
Entity Detected: | Wnt10b, wingless related MMTV integration site 10b ( MGI:108061) |
Notes: | The Wnt10b clone used as probe template in this study was obtained from A. McMahon, who in turn obtained it from Phil Leder. It is described as a 3.2kb fragment cloned into in pBSII SK using EcoRI sites.
The insert sequence of the template clone has been verified by Summerhurst and Murphy and extends 5' from nt294 in NM_011718.1, yet it also contains extra 5' and 3' sequence which both align with the genomic sequence NW_001030577.1. Total insert size is approximately 3.2kb. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |