Type: | in situ hybridisation probe |
Identifier: | Wnt16 probeA |
Entity Detected: | Wnt16, wingless-related MMTV integration site 16 ( MGI:2136018) |
Sequence: | sense strand is shown
>Wnt16 probeA
ACGAGAGGTGGAACTGTATGGTCGCCACTACCACTTCCACCCAGCTCGCCACAGCCCCCCTCTTTGGCTA
TGAGCTGAGTAGCGGCACCAAGGAGACAGCATTCATTTATGCCATCATGGCAGCGGGCCTGGTGCACTCT
GTCACCAGGTCATGCAGTGCAGGCAACATGACCGAATGTTCCTGTGAAACCACCTTGCAGAATGGTGGCT
CACCAAGTGAAGGCTGGCACTGGGGAGGATGCTCGGATGATGTCCAGTACGGCATGTGGTTCAGCAGAAA
GTTTCTAGATCTTCCCATCAGAAACACCACAGGAAAAGAAAGCAGAGTCCTGCTAGCCATGAATCTACAC
AACAACGAAGCGGGGCGGCAGGCTGTCGCCAAGTTAATGTCTGTGGACTGCCGCTGCCACGGAGTTTCCG
GCTCCTGTGCTGTGAAAACCTGCTGGAAAACTATGTCTTCTTTTGAAAAGATTGGGCATTTTTTAAAGGA
TAAATATGAAAACAGCATCCAGATCTCAGACAAAACCAAGAGGAAAATGCGCAGGAGAGAAAAAGACCAG
AGGCAGACCCCCATTCTCAAGGATGACTTGCTGTACGTTCATAAGTCTCCCAACTACTGCGTGGAGAACA
AGAAACTGGGGATTCCTGGGACCCAGGGCAGAGAGTGCAACCGGACATCAGGAGGCGCAGATGGCTGTAA
CCTCCTCTGCTGTGGCCGAGGCTACAACACCCATGTAGTCAGGCACGTGGAGAGGTGTGAGTGTAAGTTT
ATCTGGTGCTGCTACGTCCGCTGCAGGAGGTGTGAAAGTATGACCGATGTCCACACGTGTAAGTAACCTC
TCCGTCCAGCCTAGCATGAGACGCCTCTGTAGTAACCAAGGTGTGGTGTTGGCATCTGGAGGGCGCCCCT
ACTGTGCACTGATGGGGAAGTCGCTGCCTGTAAGAGTGTTCCCAGACCCCTGGGCTAGTCTACGATTTCT
TTCTTTCTGGCAGGC
|
| nt 537 - nt 1531 of NM_053116.3 |
Notes: | The Wnt16 probe used in this study was generated by RT-PCR using the following primers: forward: 5'-ACGAGAGGTGGAACTGTATGG-3'; reverse: 5'-GCCTGCCAGAAAGAAAGAAATCG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). The probe was transcribed from the entire insert. |
Chemistry: | RNA |
Strand: | antisense |
Label: | digoxigenin |