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EMAGE:5757

Lef1 lymphoid enhancer binding factor 1 ( MGI:96770)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:5757 EMAGE:5757 EMAGE:5757 EMAGE:5757 EMAGE:5757
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:5757Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
5757_voxel_strong_3D_1.wlz
5757_voxel_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5757_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Lef1 probeA
Entity Detected:Lef1, lymphoid enhancer binding factor 1 ( MGI:96770)
Sequence:sense strand is shown

>Lef1 probeA
TAGAGACGCTGATCCATAAAGACAATCACTGCCAACCCCTCTTTCGTCTACTGCAAGAGCCAAGTTCCAA
AATAAAGCATAAAAAGGTTTTTTAAAAGGAAATGTAAAAGCACATGAGAATGCTAGCAGGCTGTGGGGCA
GCTGAGCAGCTTTTCTCCCCTCATATCTGCGTGCACTTCCCAGAGCATCTTGCATCCAAACCTGTAACCT
TTCGGCAAGGACGGTAACTTGGCTGCATTTGCCTGTCATGCGCAACTGGAGCCAGCAACCAGCACATCCA
TCAGCACCCCAGTGGAGGAGTTCATGGAAGAGTTCCCTCTTTGTTTCTGCTTCATTTTTCTTTCTTTTCT
TTTCTCCTAAAGCTTTTATTTAACAGTGCAAAAGGATCGTTTTTTTTTGCTTTTTTAAACTTGAATTTTT
TTAATTTACACTTTTTAGTTTT
nt 2019 - nt 2460 of NM_016269.3
Notes:The template clone used to generate the Lef1 probe used in this study was obtained from J. Meeldijk. The insert sequence of the clone has been verified by Vendrell, Summerhurst and Murphy (it spans nt 2019-2460 of the Lef1 cDNA RefSeq NM_016269.3).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Victor Vendrell, Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Victor Vendrell, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE