EMAGE mouse gene expression spatial database

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EMAGE:5760

Tcf7 transcription factor 7, T-cell specific ( MGI:98507)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


3D reconstructed object showing signal.	All sections along the X-axis, as movie.	All sections along the Y-axis, as movie.	All sections along the Z-axis, as movie.	Photograph prior to 3D imaging.

View 3D opt image:
Download 3D images in woolz format
Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

View mapped 3D expression image

Download individual expression domains:

5760_voxel_strong_3D_1.wlz

5760_voxel_possible_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:5760_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Tcf7 probeA
Entity Detected:	Tcf7, transcription factor 7, T-cell specific ( MGI:98507)
Sequence:	sense strand is shown >Tcf7 probeA GGCCTGAAGGCCCCGGAGTGTGCCAGTGGCATGTACAAAGAGACTGTCTACTCTGCCTTCAATCTGCTCA TGCCCTACCCACCGGCCTCCGGAGCAGGGCAGCATCCGCAGCCTCAACCCCCGCTGCATAACAAGCCCGG CCAGCCTCCACATGGCGTCCCCCAGCTTTCTCCACTCTACGAACATTTCAGCAGTCCACACCCCACCCCT GCACCAGCGGACATCAGCCAGAAGCAAGGAGTTCACAGGCCGCTGCAGACCCCCGATCTCTCTGGATTTT ATTCTCTGACCTCAGGCAGCATGGGACAGCTCCCCCATACTGTGAGCTGGCCCAGTCCTCCTCTCTACCC CCTGTCCCCTTCCTGCGGATATAGACAGCACTTCCCTGCCCCCACTGCAGCCCCTGGCGCCCCCTATCCC AGGTTCACCCACCCATCCTTGATGCTGGGATCTGGTGTACCTGGACACCCAGCAGCCATCCCTCACCCGG CCATTGTGCCCTCCTCAGGGAAGCAGGAGCTGCAGCCATATGATAGAAACCTGAAAACACAGGCAGAACC CAAGGCAGAGAAGGAGGCTAAGAAGCCAGTCATCAAGAAACCCCTCAATGCGTTCATGCTTTACATGAAG GAGATGAGAGCCAAGGTCATTGCTGAGTGCACACTCAAGGAGAGCGCTGCCATCAACCAGATCCTGGGTC GCAGGTGGCATGCACTATCTCGAGAAGAGCAGGCCAAGTACTATGAACTGGCCCGCAAGGAAAGGCAGCT GCACATGCAGCTATACCCGGGCTGGTCCGCGCGGGATAACTACGGAAAGAAGAAGAGGCGGTCAAGGGAA AAACATCAAGAATCCACCACAGGAGGAAAAAGAAATGCATTCGGTACTTACCCGGAGAAGGCCGCTGCCC CAGCCCCGTTCCTTCCGATGACAGTGCTCTAGGCTGTCCCCGGTCCCCAGCTCCCCAGGACTCACCCTCG TATCTTCTGTTGCCCTCCTTCCCCACAGAACTGCTTGCTAGCCCAGTGGAGCCAGCACCAACATTCTCAG GTCGCTCTGCAGCTCTCACTCTTCCAGCCCCTAAGTCTCCAAAGACCCCCCACAGCACCCTCCAGAATCC ACAGATACAGCAACAAGAATCTCAGAGACAGGCAGCCTAACAGCCTTGGTTGCTAGGCACAGAACACCTG GCTTGCTCCAGGGGCCCACCCCTAACTCAGGAGGAGGCAACATGACCAGCTCCCATGGAACCAGCTAGGG GTCCTCTTAAGGTGATGAAGATTTCAAAGGCTGCACAACTCCCTGAACCAGGGGCCATCAATTCAAGAGG TGGGGGATTAGATCTATGTAGATGTATCATCTTAAGGGGATCCTGCCGCCTCTGACAGCCATATCTCTTG TTATTGCTGGCTGGACCTCCCTATTGGCCTAGGAGGTTTTGTTTGCTGTAGGTCAGATGGGTTGGACTGA TCTCTTCAGCTGCCTACCCTACAAATGCTTCTCCTGCCCTCTGCCACACCCTCCCCATGCCAATACTTCT TGGACCAAGAATACC
	nt 18 - nt 1572 of NM_009331.3
Notes:	The template clone used to generate the Tcf7 (Tcf1) probe used in this study was obtained from R.Grosschedl. The insert sequence of the clone has been verified by Vendrell, Summerhurst and Murphy (it spans nt 18-1572 of the Tcf7 cDNA RefSeq NM_009331.3).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	CD1
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	opt

Procedures

Fixation:	4% paraformaldehyde
Embedding:	agarose
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Victor Vendrell, Kristen Summerhurst, Paula Murphy
Principal investigator:	Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:	Victor Vendrell, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:	non-screen
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source