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EMAGE:5766

Tcf7l1 transcription factor 7-like 1 (T-cell specific, HMG box) ( MGI:1202876)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:5766 EMAGE:5766 EMAGE:5766 EMAGE:5766 EMAGE:5766
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: no stars
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Tcf3 probeA
Entity Detected:Tcf7l1, transcription factor 7-like 1 (T-cell specific, HMG box) ( MGI:1202876)
Sequence:sense strand is shown

>Tcf3 probeA
GACGACCTCGGAGCGAACGACGAGCTGATCCCCTTCCAGGACGAGGGGGGCGAAGAGCAAGAGCCGAGCA
GCGACAGCGCTTCGGCGCAGAGGGATCTGGATGAGGTCAAGTCGTCCCTGGTCAACGAATCGGAGAATCA
GAGCAGTAGCTCGGACTCCGAGGCGGAGAGGCGCCCGCAGCCCGCCCGGGACGCTTTCCAGAAGCCGCGC
GACTATTTCGCCGAAGTGAGAAGGCCCCAGGATGGCGCTTTTTTTAAGGGACCTGCGTATCCTGGGTACC
CCTTCCTGATGATTCCAGACCTGAGCAGCCCGTACCTCTCCAACGGACCCCTGTCTCCTGGTGGAGCGCG
CACCTACCTACAGATGAAATGGCCCCTCCTCGATGTCCCCTCCAGTGCTACAGTCAAGGACACAAGGTCG
CCATCTCCAGCACACTTGTCCAACAAAGTTCCTGTTGTTCAGCACCCTCATCACATGCACCCGCTGACAC
CTCTCATCACCTACAGCAACGACCACTTCTCCCCTGCGTCCCCTCCCACGCATCTGTCCCCAGAGATCGA
CCCAAAGACAGGAATCCCCCGGCCCCCTCATCCATCTGAGCTGTCACCGTATTACCCACTGTCTCCAGGA
GCTGTCGGACAAATCCCCCATCCCCTCGGCTGGCTCGTCCCACAGCAAGGGCAGCCCATGTACTCTCTCC
CTCCGGGTGGTTTCCGGCATCCTTACCCTGCCCTCGCCATGAACGCTTCAATGTCCAGCCTGGTCTCAAG
CCGGTTCCCACACATGGTGGCTCCTGCCCATCCTGGTCTGCCCACCTCAGGAATCCCCCACCCTGCCATC
GTCTCCCCCATTGTGAAGCAGGAGCCAGCAGCCCCCAGCCTGAGCCCTGCAGTGAGTGCGAAATCCCCAG
TTACGGTGAAGAAGGAAGAGGAGAAGAAACCTCACGTGAAAAAGCCCCTGAATGCCTTCATGTTGTATAT
GAAGGAGATGAGGGCCAAGGTGGTGGCCGAGTGTACCCTGAAGGAAAGTGCAGCCATTAACCAAATCCTG
GGAAGAAAGTGGCACAACCTGTCAAGAGAAGAACAGGCCAAATACTATGAGCTTGCCCGGAAAGAACGGC
AGCTTCACGCGCAGCTCTACCCAACCTGGTCAGCCCGGGACAACTATGGGAAGAAGAAGAAGAGGAAGAG
AGAAAAGCAGCTGTCACAGACACAGTCTCAGCAGCAAATCCAAGAGGCAGAGGGTGCTCTGGCCTCTAAG
AGCAAGAAGCCATGCATTCAGTACCTGCCCCCTGAGAAGCCTTGTGATAGCCCTGCGTCTTCCCATGGCA
GCACGCTGGACTCCCCTGCGACGCCCTCCGCAGCCTTGGCCTCACCAGCTGCCCCTGCGGCTACCCACTC
CGAGCAAGCGCAGCCCCTGTCGCTCACCACCAAACCGGAGGCCCGGGCCCAGCTGGCTCTCCACTCCGCT
GCCTTCCTGTCGGCTAAGGCGGCCGCC
nt 86 - nt 1582 of NM_009332.2
Notes:The template clone used to generate the Tcf3 probe used in this study was cloned from a "limb library screen".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Victor Vendrell, Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Victor Vendrell, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE