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EMAGE:6158

Wnt9b wingless-type MMTV integration site 9B ( MGI:1197020)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6158 EMAGE:6158 EMAGE:6158 EMAGE:6158 EMAGE:6158
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:6158Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
6158_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6158_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt9b probeA
Entity Detected:Wnt9b, wingless-type MMTV integration site 9B ( MGI:1197020)
Sequence:sense strand is shown

>Wnt9b probeA
TCCTTCTCTATGACCCTAATCCTGCTCTGGCTCGAGGGTACAAGGCAAGAATGGAGCCTGGCAAAACTTG
GGGACTAGAACACCTGGACCTACAGCCAAATCACCTGTACCCTGACTCTATGGCCAGGAGGGCCAGGGGT
GGAGGAGGGTTAAAGATGAACTTGAAGTTGAGGGCTGAGGCTGACCAACCATTAAGACTGGTGCCTTAAG
GCACCCTCAGTCAGGTCCTCTCCCTCCCTTCTCCATTCTTTCTCCAAGGCCCCGTTCCCCCTAAAATCCC
ACCATAGCCATGCTGGGTCCCCCCTTCCCCCACACTGGGAACTTTAAGGAAGATATTCACAGGGTATTTC
TGCCTACCTCATACATGTAATTTTCAAAAAAAATTAATTTATATAGTTAAGATATATGGGAAAGTATTTA
TGTTATTTATATATCTTCTCTATTTCCTGGGCACCATATGGGGGGTTGTGTGTTTACCCAGAAGCCTCTG
AGGAAACATGGCTGGGTCTGTCTGGGGCCTCGCAGAGCTGGATGCGCATAGCTGAGAGGTCACAGCTCCT
GTGTCTCACTGTCTTGGAGCTCGGGAAGCACATGTACCTCCTGAGATAAACCCCGTGACACCAA
nt 2833 - nt 3456 of NM_011719.3
Notes:The Wnt9b probe template used in this study was generated by RT-PCR using the following primers: forward: 5'-TCCTTCTCTATGACCCTAATCCTGC-3'; reverse: 5'-TTGGTGTCACTTTTTTTTATCTC-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). The probe was transcribed from the entire insert.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE