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EMAGE:3368

Hoxb4 homeobox B4 ( MGI:96185)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3368 EMAGE:3368 EMAGE:3368 EMAGE:3368
Figure 6A of Brend et al., 2003 [PMID:12736215] . Copyright: This image is from Brend et al., Development 130, 2717-2728 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 6B of Brend et al., 2003 [PMID:12736215] . Copyright: This image is from Brend et al., Development 130, 2717-2728 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 6C of Brend et al., 2003 [PMID:12736215] . Copyright: This image is from Brend et al., Development 130, 2717-2728 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 6D of Brend et al., 2003 [PMID:12736215] . Copyright: This image is from Brend et al., Development 130, 2717-2728 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: (A,B,E,F) Black arrowheads, somites 7-13; white arrowhead, rhombomere 6/7 boundary. (D) White arrow, tailbud.
Expression Pattern Description
Spatial Annotation:
EMAGE:3368Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3368_wholemount_strong_3D_1.wlz
3368_wholemount_moderate_3D_1.wlz
3368_wholemount_weak_3D_1.wlz
3368_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3368_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rhombomere 07
detected detected
Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
rhombomere 08
detected detected
Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
neural tube
detected detected
Expression throughout neural tube from the rhombomere 6/7 boundary to the posterior tip of the embryo. Authors state that staining was often weaker in the interlimb
somite 07
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 08
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 09
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 10
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 11
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 12
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
somite 13
detected detected
Expression in somites 7 to 13, but not in more posterior somites.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2677499
Entity Detected:Hoxb4, homeobox B4 ( MGI:96185)
Notes:The Hoxb4 probe used in this study by Brend et al., 2003 [PMID:12736215] "was provided by R. Krumlauf (NIMR, Mill Hill)". Editor's Note: The nucleotide sequence of the probe is unspecified by the authors.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:Mirsky's fixative
General Information
Authors:Brend T; Gilthorpe J; Summerbell D; Rigby PW, 2003 [PMID:12736215] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.00471] [ PMID:12736215] Brend T, Gilthorpe J, Summerbell D, Rigby PW 2003 Multiple levels of transcriptional and post-transcriptional regulation are required to define the domain of Hoxb4 expression. Development (130):2717-28
Links:MGI:2677719 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI